Virus-inclusive single cell RNA sequencing reveals the molecular signature of dengue virus infection in cell lines and human blood
Speaker: Fabio Zanini
Department of Bioengineering
Dengue virus (DENV) infects millions of people annually and can cause severe manifestations including hemorrhage and shock. However, a thorough understanding of the host response to DENV is lacking, partly because conventional approaches ignore heterogeneity in virus abundance across cells. In this talk, I will introduce viscRNA-Seq (virus-inclusive single cell RNA-Seq), an approach to probe the host transcriptome together with intracellular viral RNA at the single cell level. I applied viscRNA-Seq to monitor DENV infection in cultured cells and peripheral blood cells from dengue infected human subjects.
Longitudinal sampling of cultures cells enabled the identification of host factors that play pro- or antiviral roles. In blood cells from dengue patients, interferon response genes were upregulated in a cell-specific manner prior to severe symptoms. The majority of DENV-RNA containing cells were naive IgM B cells expressing the CD69 and CXCR4 receptors and antiviral genes, followed by monocytes. Unexpected patterns of genetic diversity were detected in the viral genome and the host antibody repertoire. ViscRNA-Seq enables a multi-faceted molecular elucidation of viral infection in cell lines and human tissues and the discovery of novel virus-host genetic interactions.
Streaming video: the seminar will be broadcast live once the event has started. The video recording may also be viewed at a later date. [link]
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